Standard Operating Procedure
Anasazi EFT FT-NMR
Nuclear Magnetic Resonance

NMR Setup and Operation Instructions
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Created by-
Dr. Fred O, Garces
Chemistry Department
Miramar College

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	Precautions • If you wear a pacemaker, STOP, you are not allowed to use this instrument. The magnet may affect your pacemaker. • Remove watches or electronic devices on your person, these may get demagnetize. • Do not handle spinner with your bare hands, always use Kimwipe® to handle the NMR spinner and clean any finger residue from NMR sample before inserting in magnet. • Clean the NMR area when you finish using the instrument.
	Quick Overview I. Prepare the Sample sample     A. Use NMR tube with appropriate sample.       II. Turn on Spectrometer and Inserting Sample   A. In PNMR, run D2O sample to optimize spin rate & shim magnet.     III. Preparing Spectrometer to suppress spinning sidebands   A. Enter chemical shift of water   B. Adjust spin rate so FID shows crest-to-crest of 5 division   C. Adjust spin rate so FID shows crest-to-crest of 2 division     IV Acquire NMR spectrum   A. Adjust shim and gain to sample   B. Set up field offset freq so TMS is at 0 PPM   C. Collect data for NMR sample, 1H or 13C       Be sure number of scan (ns) is appropriate for good S/N     V. Process and plot the data   A. In NUTS zoom in to region of interest   B. Phase spectrum (AP) and apply base line correction (BC)   C. Integrate the 1H NMR spectrum and assign integration value.   D. Pick peaks and label chemical shift   E. Plot spectrum
	PDF References I. Quick Start     A. Anasazi Quick Start Guide   B. EFT Quick Start.      II. EFT Spectrometer Guide   A. EFT Spectrometer Guide     III. NUTS, NMR data processing software   A. NUTs, for Windows 98 and Mac (2004)   B. NUTs, for Windows 3.1 (2000)     IV Shimming   A. Shimming Magnets, Fudnamentals     V. Tutorials   A. Other SOP for instrument   B. Basic PNMR Tutorial   C. PNMR Liquid Samples

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