The maximum and zero absorbance values must be set each time the wavelength is changed.
Wipe the outside of the cuvet containing the sample and place it in the sample compartment. Remember to align the marks. Read and record the %T and convert to absorbance.
Click on the sample below which is appropriate for use in the Spectronic 20™...
The bubbles present in this test tube will cause any measurements to be inaccurate.
This cuvet has not been filled with enough of the solution. Some light may not pass through the sample causing the measurement to be inaccurate.
There is a crack in the cuvet. The crack will cause your measurement to be inaccurate.
This cuvet is appropriate for use. There ar no bubble, cracks, or fingerprints on this cuvet, and it has been filled with enough solution.
If you look closely at the cuvet, you can see a fingerprint on the cuvet. The fingerprint will cause your measurement to be inaccurate.
Click on the sample below which is appropriate for use in the Spectronic 20™...
