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Turn the Instrument On: Turn the instrument on using the Power Switch/Zero Control knob, and allow the Spectronic 20™ to warm up for at least 15 minutes before collecting any data with a Spectronic 20™.
Prepare Solutions:
A quantitative spectroscopic analysis requires quantitative (analytical) solutions. Review the use of the pipets (Mohr or volumetric), volumetric flask, and graduated cylinder web pages for the preparation of analytical solutions.
Select an Appropriate Cuvet: A cuvet must not absorb light in the region being investigated (see Table 1). For a Spectronic 20™, a cuvet is often shaped like a test tube, but for more accurate results a square cuvet could be used.
Table 1.
Transparent regions for common cuvet material
(Data from Fisher Catalog P.1745-46, 1995/96)
| material | | range
|
|---|
| pyrex | 350-2500 nm |
| fused silica | 190-3600 nm |
| plastic | 300-800 nm |
|
