Spectronic 20™

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Description
Preparing to Use a Spectrometer
About the Meter
Reading the %T from the Meter
Setting the Wavelength
Setting Maximum Absorbance / 0% Transmittance
Setting Zero Absorbance / 100% Transmittance
Preparing the Cuvet and Placing the Sample in the Spectrometer
Measuring the %Transmittance
Additional Topics
Colors
Recording an Absorption Spectrum with a Spectronic 20™
Self Check Exercises 1
Self Check Exercises 2
Related Modules
Spectrometer, Scanning
Spectroscope, Hand-held
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Setting the Wavelength

 

IMAGE. A labeled Spectronic 20™

The wavelength is selected by rotating the wavelength control knob until the desired wavelength is displayed in the window next to the control knob.

On instruments that are used often, there is occasionally play in the wavelength control knob (the knob is loose). Thus, the absorbance read from the meter may not be the same for the same solution if the wavelength is changed between measurements. The different readings are due to slight differences in the position of the grating (therefore different wavelengths of light are passed through the sample) after moving the wavelenght control knob.

One method to reduce this error is to only turn the wavelength control knob one in direction when adjusting the wavelength. If the desired wavelength is missed on the first attempt, return the control well before the desired wavelength and attempt to set the wavelength again.