Spectronic 20™

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Description
Preparing to Use a Spectrometer
About the Meter
Reading the %T from the Meter
Setting the Wavelength
Setting Maximum Absorbance / 0% Transmittance
Setting Zero Absorbance / 100% Transmittance
Preparing the Cuvet and Placing the Sample in the Spectrometer
Measuring the %Transmittance
Additional Topics
Colors
Recording an Absorption Spectrum with a Spectronic 20™
Self Check Exercises 1
Self Check Exercises 2
Related Modules
Spectrometer, Scanning
Spectroscope, Hand-held
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About the Meter

 

IMAGE. The meter on a Spectronic 20™ viewed such that parallax has been eliminated.


IMAGE. Note the reflection in the mirror and that a reading of 58.1 %T would be recorded. This value would be inaccurate due to the incorrect positioning when the reading was made.

The Two Scales: The Spectronic 20™ meter displays both absorbance and % transmittance. Readings from the meter should always be taken from the % transmittance scale since it is more difficult to estimate between the graduation marks on the logarithmic, absorbance scale. Percent Transmittance can be converted to absorbance using the following relationship:

A = -log (%T/100) = log 100 - log %T

The Mirror Behind the Needle: When looking at the meter, eliminate the reflection of the needle in the mirror. When the reflection is eliminated, the viewer is directly in front of the meter and parallax has been eliminated. Correct and incorrect views of the meter are shown.